goldbrick\nOver time, peritoneal dialysis results in functional and structural alterations of the peritoneal membrane, exactly the underlying mechanisms and whether these changes atomic number 18 reversible are non completely understood. Here, we studied the personal effects of spicy levels of glucose, which are ensnare in the dialysate, on military man peritoneal mesothelial cells (HPMCs). We found that towering assimilations of glucose have gotd epithelial-to-mesenchymal transition (EMT) of HPMC, suggested by reduced expression of E-cadherin and change magnitude expression of alpha-smooth muscle actin, fibronectin, and emblem I collagen and by change magnitude cell migration. Normalization of glucose concentration on day 2 reversed the phenotypic transformation, but the changes were irreversible after 7 d of stimulation with senior high school glucose. In addition, exposure of HPMC to high glucose resulted in a lessen expression of the antifibrotic cytokines, hepatocy te growth factor (HGF) and bone morphogenic protein 7 (BMP-7). exogenic treatment with HGF resulted in a dosage-dependent prevention of high glucose-induced EMT. two BMP-7 peptide and gene transfection with an adenoviral vector of BMP-7 in addition protected HPMCs from EMT. Furthermore, adenoviral BMP-7 transfection decreased peritoneal EMT and ameliorated peritoneal thickening in an tool model of peritoneal dialysis. In summary, high concentrations of glucose induce a reversible EMT of HPMCs, associated with decreased production of HGF and BMP-7. Treatment of HPMCs with HGF or BMP-7 blocks high glucose-induced EMT, and BMP-7 ameliorates peritonealfibrosis in an animal model of peritoneal dialysis.If you insufficiency to get a wide-cut essay, order it on our website:
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